In Situ Proximity Ligation Assay - In Situ PLA

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In 2006, Ola Soderberg and colleagues established a technique called in situ proximity ligation assay (in situ PLA) to reveal protein-protein interactions in cells. The technique recognizes a target protein by binding a "probe" consisting of a pair of proteins attached to DNA onto the target protein.

Soderberg and colleagues developed a new general version of the technique in which different probes can identify proteins that have undergone various changes in their structure.

The researchers used this technique to detect a protein on the membrane of cells called platelet-derived growth factor receptor beta, which undergoes changes that will promote cell proliferation and movement.

References:
"In Situ Detection of Phosphorylated Platelet-derived Growth Factor Receptor Beta Using a Generalized Proximity Ligation Method"

Malin Jarvius, Janna Paulsson, Irene Weibrecht, Karl-Johan Leuchowius, Ann-Catrin Andersson, Carolina Wahlby, Mats Gullberg, Johan Botling, Tobias Sjoblom, Boyka Markova, Arne Ostman, Ulf Landegren, and Ola Soderberg.
Molecular & Cellular Proteomics, Sept. 2007

Will love this method...