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RNAi and SiRNA Forum Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.


What are the uses of RNAi in the different cell types?

What are the uses of RNAi in the different cell types? - RNAi and SiRNA Forum

What are the uses of RNAi in the different cell types? - Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.


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Old 05-15-2013, 12:16 PM
Pipette Filler
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Default What are the uses of RNAi in the different cell types?



Can RNAi mechanism protect us from jumping-repetitive elements and from genomic inbalance?
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Old 05-15-2013, 12:16 PM
Pipette Filler
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RNAi is a single stranded RNA molecule that is complementary to and binds an mRNA molecule. This sets in motion a sequence of events that destroys the entire dsRNA: dicer first chops up the dsRNA into little fragments, then the fragments are broken down in the RNA induced silencing complex (RISC).

So, you're talking about LINEs and SINEs (long/short interspersed nuclear elements). LINEs are basically a copy and past mechanism in that they encode a ribosome; SINEs are cut and paste and do not make a copy of themselves. So, if you could sequence these elements and produce a complementary copy, I don't see why you couldn't use RNAi to stop their jumping around. However, why would you want to? They generally don't cause any problems. In fact, they're useful in terms of defining evolutionary relatedness and constructing phylogenetic trees.

I don't know what genomic imbalance is. . .apologies.
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Old 05-15-2013, 12:16 PM
Pipette Filler
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RNA interference is the process where a double stranded piece of RNA is destroyed and any single stranded RNA, including mRNA, with a similar sequence is also destroyed. This allows for a specific gene to be silenced so scientists now use it as a tool for gene knock downs, although it was probably first used in nature as a defence against viruses that use dsRNA . Yes it is also used to defend against jumping-repetitive elements, more commanly known as transposable elements.
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Old 05-15-2013, 12:16 PM
Pipette Filler
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It's a technique to disable a gene or stop DNA from being expressed by using RNA interference. I don't see how it can be used to protect us from the things you suggested but who knows in the future what this biotechnology will develop into.
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