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-   -   Does the siRNA/dsRNA come from same gene as mRNA in RNA interference (RNAi)? (http://www.molecularstation.com/forum/rnai-sirna-forum/87755-does-sirna-dsrna-come-same-gene-mrna-rna-interference-rnai.html)

Shannon 03-30-2013 06:18 AM

Does the siRNA/dsRNA come from same gene as mRNA in RNA interference (RNAi)?
 
I am kind of confused about this question. I understand that the sequence of the double stranded RNA must be complimentary to the sequence on the messenger RNA it aims to affect. But in my writing my biology teacher asked me if siRNA and/or dsRNA come from same gene as the mRNA. I have looked all over for this and do not understand what she means. Any help would be great. If feeling super ambitious, direction to a peer reviewed journal referencing this in a sentence or two would be greatly appreciated. Thank you.

Jon Moulton 04-01-2013 02:57 PM

Re: Does the siRNA/dsRNA come from same gene as mRNA in RNA interference (RNAi)?
 
In many cases miRNAs are encoded on different genes; since a single miRNA may have hundreds of mRNA targets, the majority of miRNA-mRNA interactions are between RNAs encoded from different loci.

I recall running across a reference to an autoregulatory miRtron. This is an miRNA encoded in an intron of a gene; they are transcribed together, the intron is spliced out and the miRNA sequence is processed to a mature miRNA, which then suppresses translation of the mRNA with which it was transcribed. I don't recall the reference, I suppose I'm not ambitious enough to hunt.


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