| | Re: What is RNA silencing..???????????? Wrt to RNA interference?
You have a peptide, say WT1, that is being expressed a lot in a given cell line, but you want to silence the expression of that peptide. Once you know the mRNA sequence of WT1, you order an antisense oligonucleotide, complementary to a part of WT1's mRNA sequence. Next, you introduce that oligo in your cell line by the method you see fit (eletroporation, etc.).
Once in the cytoplasm of the cells of interest the oligo will bind to WT1 mRNA, a part of this will become double stranded impeding its further translation. That will silence the expression of WT1 as long as there are antisense oligo in that cell's cytoplasm. That is RNA interference.