| | Re: RNA Interference topic using Agrobacterium?
A single stranded RNA with some self-complementary sequence folds back on itself to form a hairpin structure. The structure is cut by two nucleases (commonly drosha and dicer) to form a short duplex of RNA with a few overhanging single-stranded bases at either end. The duplex is loaded onto RISC and one strand is cut. The cut strand dissociates, leaving a single strand loaded into RISC and ready to interact with RNAs.
When RISC encounters RNAs completely complementary to the mIRNA strand, it cleaves the RNA. When it encounters partially-complementary targets, RISC can bind
Last edited by Jon Moulton; 01-08-2013 at 08:48 PM.