RNA Interference topic using Agrobacterium?

[vandana]

Its so confusing how the RNA interference works with the whole single rna becoming double stranded.

and whats the net effect?

PLEASE help and EXPLAIN!!

[Jon Moulton]

A single stranded RNA with some self-complementary sequence folds back on itself to form a hairpin structure. The structure is cut by two nucleases (commonly drosha and dicer) to form a short duplex of RNA with a few overhanging single-stranded bases at either end. The duplex is loaded onto RISC and one strand is cut. The cut strand dissociates, leaving a single strand loaded into RISC and ready to interact with RNAs.

When RISC encounters RNAs completely complementary to the mIRNA strand, it cleaves the RNA. When it encounters partially-complementary targets, RISC can bind

[Jon Moulton]

When RISC encounters RNAs completely complementary to the miRNA strand, it cleaves the RNA. When it encounters partially-complementary targets, RISC can bind to the RNA and inhibit translation of the RNA.