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-   -   RNA Interference topic using Agrobacterium? (http://www.molecularstation.com/forum/rnai-sirna-forum/87277-rna-interference-topic-using-agrobacterium.html)

vandana 01-05-2013 06:27 AM

RNA Interference topic using Agrobacterium?
 
Its so confusing how the RNA interference works with the whole single rna becoming double stranded.

and whats the net effect?

PLEASE help and EXPLAIN!!

Jon Moulton 01-08-2013 08:47 PM

Re: RNA Interference topic using Agrobacterium?
 
A single stranded RNA with some self-complementary sequence folds back on itself to form a hairpin structure. The structure is cut by two nucleases (commonly drosha and dicer) to form a short duplex of RNA with a few overhanging single-stranded bases at either end. The duplex is loaded onto RISC and one strand is cut. The cut strand dissociates, leaving a single strand loaded into RISC and ready to interact with RNAs.

When RISC encounters RNAs completely complementary to the mIRNA strand, it cleaves the RNA. When it encounters partially-complementary targets, RISC can bind

Jon Moulton 01-08-2013 08:49 PM

Re: RNA Interference topic using Agrobacterium?
 
When RISC encounters RNAs completely complementary to the miRNA strand, it cleaves the RNA. When it encounters partially-complementary targets, RISC can bind to the RNA and inhibit translation of the RNA.


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