Substitute siRNA with DNA or PNA
I have got a question: Let's suppose we have a working siRNA. What happens when we substitute the RNA by a corresponding DNA or PNA sequence?
I am new to the siRNA-field, but what I have read so far led me to assume that neither one of the substitutes could be processed by the DICER-complex. So in the end, a the corresponding DNA or PNA sequences would only work as anti-sense oligos, and most likely lose most of its effectiveness as compared to siRNA, right?
Re: Substitute siRNA with DNA or PNA
I agree. The mechanism of action of these oligos is entirely different, neither the DNA nor the PNA would activate argonaute-mediated nucleolytic activity. DNA would activate RNase-H for nucleolytic cleavage of target RNA, while the PNA is steric-blocking antisense.
|All times are GMT. The time now is 09:26 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved