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RNAi and SiRNA Forum Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.


Higher mRNA level after RNAi?

Higher mRNA level after RNAi? - RNAi and SiRNA Forum

Higher mRNA level after RNAi? - Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.


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Old 02-12-2012, 07:00 PM
Pipette Filler
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Default Higher mRNA level after RNAi?



Hi everyone,
I'm rather new to this technique and am trying to test the function of a gene using RNAi in vivo. Iíve fed my insect with dsRNAs and found expected decrease in enzymatic activities of the gene. What confuses me is when I tested the mRNA level using rt-PCR. There was actually higher amount of mRNA of the gene. I've read some references and most of them said that the expected results should be reduced mRNA levels, which is in conflict with the enzymatic activity I'm observing. I was wondering if it is possible that the cells are trying to compensate the product and are upregulating the expression of the gene?

Thanks.
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