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development of siRNA Transfection reagent!!!!!!!!

development of siRNA Transfection reagent!!!!!!!! - RNAi and SiRNA Forum

development of siRNA Transfection reagent!!!!!!!! - Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.


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Old 08-09-2011, 01:52 PM
Pipette Filler
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Default development of siRNA Transfection reagent!!!!!!!!



Hi everybody;
1 want to ask that I try to developing lipidic transfection reagent. but When my reagent normally bind to siRNA, it is release the siRNA in culture medium. so transfection does not happen what can I do ?
Does it matter whether or not HEPES buffer in transfection reagent or culture medium? and also is sodium bicarbonate concentration impotant? for example ı use the 3.7g/L sodium bicarbonate for normal cell culture and also in culture medium for transfection.
thanks
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