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-   -   development of siRNA Transfection reagent!!!!!!!! (http://www.molecularstation.com/forum/rnai-sirna-forum/83320-development-sirna-transfection-reagent.html)

Behiye 08-09-2011 01:52 PM

development of siRNA Transfection reagent!!!!!!!!
 
Hi everybody;
1 want to ask that I try to developing lipidic transfection reagent. but When my reagent normally bind to siRNA, it is release the siRNA in culture medium. so transfection does not happen what can I do ?
Does it matter whether or not HEPES buffer in transfection reagent or culture medium? and also is sodium bicarbonate concentration impotant? for example ı use the 3.7g/L sodium bicarbonate for normal cell culture and also in culture medium for transfection.
thanks :confused:


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