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RNAi and SiRNA Forum Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.


RNA interference :: Discovery of mechanism??? PLEASE HELP!!!?

RNA interference :: Discovery of mechanism??? PLEASE HELP!!!? - RNAi and SiRNA Forum

RNA interference :: Discovery of mechanism??? PLEASE HELP!!!? - Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.


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Old 09-24-2009, 09:16 PM
Pipette Filler
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Default RNA interference :: Discovery of mechanism??? PLEASE HELP!!!?



i know that andrew fire and craig mello discovered that dsRNA cause RNA interference but they could not deduce RNAi Pathway.( involving Dicer, RISC complex etc)

1) Can u please tell me who / which group/s deduced the RNAi pathway ( for bith si RNA and mi RNA)??

2) or can u plese type link/ site for RNAi PATHWAY discovery (that includes scientists name and their experiments to deduce the pathway)???

3) or can u type the site where i can freely download/ view the total and original paper/s of the scientists who discovered the pathway??


HELP PLEEEEEEASE!!!!!!!!!!!!!!!!!!!!!! thank u!!
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Old 09-24-2009, 09:16 PM
Pipette Filler
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First recognized by Rich Jorgenson and his team in plants (Napoli et al., 1990******), the discovery of RNAi was the unexpected result of attempts to make the colour of petunia blooms more purple. An experiment was designed to overexpress the gene responsible for the production of anthocyanin pigments (chalcone synthetase (CHS)) through the introduction of a CHS transgene attached to a powerful promoter sequence. However, instead of increasing anthocyanin levels and therefore the deepness of bloom hue, the transgenic model showed apparent silencing of both the endogenous and exogenous CHS genes, and the flowers appeared variegated or white.
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