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siRNA transfection problems-Min6 cells

siRNA transfection problems-Min6 cells - RNAi and SiRNA Forum

siRNA transfection problems-Min6 cells - Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.


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Old 09-11-2009, 09:04 AM
Pipette Filler
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Default siRNA transfection problems-Min6 cells



Hi,

I started working on Min6 cells and I tried to transfect these cells with siRNAs by using different transfection reagents such as Lipofectamine 2000, Lipofectamine RNAiMAX, Fugene6 and also Gencarrier2. But, all the attempts turned futile. The siRNAs are working really well in another cell type from the same species., mouse. Awaiting to get some clues from someone who worked with these cells.

Thanks.
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Old 04-26-2010, 08:03 AM
Pipette Filler
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Default Re: siRNA transfection problems-Min6 cells

Hey, Did you resolve the issues concerning transfection of MIN6 cells?
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cells , problemsmin6 , sirna , transfection


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