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RNAi and SiRNA Forum Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.


What is RNAi screening?

What is RNAi screening? - RNAi and SiRNA Forum

What is RNAi screening? - Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.


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Old 08-04-2009, 04:16 AM
Pipette Filler
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Default What is RNAi screening?



Can someone explain what RNAi screening is?
What is it used for?
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Old 08-04-2009, 04:16 AM
GIL GIL is offline
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To make it simple RNA interference is a single strand of RNA. Since for the most part RNA is single stranded unlike the double stranded DNA, an RNA interference is complimentary to a part of an RNA strand. Therefore, when the RNAi attaches itself to part of the RNA it can "silence" the RNA meaning that the portion of RNA that is now double stranded thanks to the attaching of the RNAi can either be inactivated or activated. Therefore, RNAi can also be used for screening RNA similar to antibodies used to screen for certain proteins.
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