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RNAi and SiRNA Forum Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.


siRNAs shRNAs microRNAs which to order?

siRNAs shRNAs microRNAs which to order? - RNAi and SiRNA Forum

siRNAs shRNAs microRNAs which to order? - Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.


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Old 05-20-2009, 01:57 PM
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Default siRNAs shRNAs microRNAs which to order?



Can anyone explain which are important for knockdown experiments to order from the following list?

1. Mimics
2. Inhibitors
3. custom or validated siRNA
4. Pre-designed siRNA set (3-4 siRNAs) with guaranteed silencing.
5. One custom or validated shRNA.
6. Pre-designed shRNA vector set (4 shRNAs) with guaranteed silencing.
7. Positive control for siRNAs
8. Negative control for siRNAs.
9. FAM-labeled negative control for siRNAs.
10. Controls for shRNAs.
11. Negative controls for mimics or inhibitors
12. FAM-labeled negative controls for mimics or inhibitors.
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