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-   -   What is the Difference Between the Different RNAi Methods? (http://www.molecularstation.com/forum/rnai-sirna-forum/3551-what-difference-between-different-rnai-methods.html)

cor 07-08-2008 06:54 PM

What is the Difference Between the Different RNAi Methods?
 
Hello,
What is the Difference Between the Different RNAi Methods?

I mean why siRNA, shRNA, miRNA, can anyone explain the difference please?

thanks

MARGARITA MARTINEZ 07-09-2008 01:36 AM

Re: What is the Difference Between the Different RNAi Methods?
 
Please Anyone Will Explain The Diference กกกกกก I Am Interesting Too

Jon Moulton 07-09-2008 02:35 PM

Re: What is the Difference Between the Different RNAi Methods?
 
OK, I'll give this a try.

miRNA is a naturally-produced molecule. Cells transcribe pri-miRNA, long strands with duplex stem-loop regions. In the nucleus, these molecules are cleaved by a protein complex involving Drosha and an RNA hairpin is released, called pre-miRNA. The hairpin is exported to the cytosol where it is cleaved by Dicer, releasing the loop and leaving a double-stranded short RNA of about 21 bases per strand with slightly overhanging single-stranded ends. The duplex is loaded into a protein complex including Argonaute, which cleaves one of the strands. The cleaved strand fragments dissociate from the newly formed RNA-protein complex. The remaining single strand binds to various nearly-complementary RNA targets, inhibiting the translation of those targets or, in cases of near-perfect or perfect complementarity, cleaving the target strand.

siRNA is an artificial molecule of short double-stranded RNA that is either directly introduced into cells or expressed in cells where it matures (by Drosha and Dicer activity) to form the short double-stranded RNA that is loaded onto Argonaute. siRNA can be introduced into cells as a double-stranded short RNA of about 21 bases per strand with slightly overhanging single-stranded ends (like the mature miRNA before loading onto Argonaute). Once in cells the siRNA loads onto Argonaute, loses a strand and functions like miRNA, either repressing translation of some partially-complementary mRNA targets or cleaving RNA regions of near-perfect complementarity.

If siRNA is introduced into a cell as a hairpin, it must be cleaved by Dicer before loading onto Argonaute. This form is called short hairpin RNA, or shRNA.

Please, folks, don't let this stand unvetted. I'd like to learn something ...

MARGARITA MARTINEZ 07-13-2008 04:23 AM

Re: What is the Difference Between the Different RNAi Methods?
 
Jon Thank You For You Explain ...

aftabuom 08-28-2008 12:03 PM

Re: What is the Difference Between the Different RNAi Methods?
 
Hi Guys,
I want to know that which is the most successfull way of transfecting mammalian cell line.........


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