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Problems getting Knock-down using SiRNA

Problems getting Knock-down using SiRNA - RNAi and SiRNA Forum

Problems getting Knock-down using SiRNA - Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.



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Old 02-21-2006, 05:55 PM
Pipette Filler
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postdock RSS Feed
Arrow Problems getting Knock-down using SiRNA
Hi,
I am having problems knocking-down a gene using SiRNA.
How do I know it is a siRNA transfection efficiency problem or a problem with targeting?
thanks
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  #2 (permalink)  
Old 02-22-2006, 06:10 PM
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clokke76 RSS Feed
Default re: sirna knockdown problems
Quote:
Originally Posted by postdock
Hi,
I am having problems knocking-down a gene using SiRNA.
How do I know it is a siRNA transfection efficiency problem or a problem with targeting?
thanks
I would check the mRNA levels of the gene you are trying to knock down.
Also, is there some type of transfection efficiency control you can do? is your siRNA in a vector? I would also try another cell line/tranfection reagent just to see if you get better knock down in another model.

some hints... anyone else?
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