Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > RNA Techniques Forum > RNAi and SiRNA Forum
Register Search Today's Posts Mark Forums Read

RNAi and SiRNA Forum Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.


Stable siRNA transfection

Stable siRNA transfection - RNAi and SiRNA Forum

Stable siRNA transfection - Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 04-20-2008, 09:57 PM
Pipette Filler
Points: 996, Level: 17 Points: 996, Level: 17 Points: 996, Level: 17
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Oct 2007
Posts: 7
Thanks: 0
Thanked 0 Times in 0 Posts
Default Stable siRNA transfection



HI,

I do a lot of siRNA transfection for expts like PI staining, protein extracts, western blot etc wherein I require a lot of cells.
The main disadvantage is that I have to do all these expts post 48 hours due to the transient nature of siRNA knockdown and also use up a lot of the expnsive Lipofectamine 2000 every week.

Is there any way to stably knockdown my gene of interest in the stable ovarian cancer cell line I use, using siRNAs? Does it take a lot of time?
Please suggest with a little detail coz I cant grasp all the promoter, construct language very quickly

Also can anyone recommend any other transfection reagents they use for siRNA treatment which is good and relatively cheaper?

Thanks,
Reply With Quote
  #2  
Old 05-22-2009, 11:34 AM
Pipette Filler
Points: 11, Level: 1 Points: 11, Level: 1 Points: 11, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2009
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Stable siRNA transfection

Hi,

I have to start the sirna transfection experiments. Can anyone help me with what are the precautions i have to follow in these experiments, as RNAses are ubiquitously present.
I have some basic knowledge of transfection and created stable cell lines also.
Reply With Quote
Reply

Tags
sirna , stable , transfection


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
siRNA Transfection Reagents joey20 RNAi and SiRNA Forum 2 12-30-2010 04:40 AM
sirna transfection biology RNAi and SiRNA Forum 0 05-25-2009 08:24 AM
Stable Transfection Picking Selecting Clones dave2006 Transfection Forum 1 08-30-2008 04:37 AM
Stable Transfection with siRNA and RNAi kiki06 RNAi and SiRNA Forum 3 07-13-2007 06:21 PM
siRNA and stable transfection of stem cells nanaz RNAi and SiRNA Forum 0 04-26-2007 06:55 AM


All times are GMT. The time now is 04:12 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11644 seconds with 16 queries