I do a lot of siRNA transfection for expts like PI staining, protein extracts, western blot etc wherein I require a lot of cells.
The main disadvantage is that I have to do all these expts post 48 hours due to the transient nature of siRNA knockdown and also use up a lot of the expnsive Lipofectamine 2000 every week.
Is there any way to stably knockdown my gene of interest in the stable ovarian cancer cell line I use, using siRNAs? Does it take a lot of time?
Please suggest with a little detail coz I cant grasp all the promoter, construct language very quickly
Also can anyone recommend any other transfection reagents they use for siRNA treatment which is good and relatively cheaper?