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miRNA Single or Double Stranded?

miRNA Single or Double Stranded? - RNAi and SiRNA Forum

miRNA Single or Double Stranded? - Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.


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Old 01-09-2008, 02:43 PM
Pipette Filler
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Question miRNA Single or Double Stranded?



Is MiRNA Single or Double Stranded?
I am having an argument with a co-worked
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Old 01-09-2008, 02:55 PM
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Default Re: miRNA Single or Double Stranded?

well
miRNA are the miRNA That are manily involved in gene silencing by later making the siRNA, so they are double stranded in a way that both complementry stranded come over each other to make that double stranded.
any other suggestion.........


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aftab
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Old 01-22-2008, 09:52 PM
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Default Re: miRNA Single or Double Stranded?

Prior to associating with Argonaute, a free miRNA is a duplex. As the miRNA strand that will recognize target mRNAs is loaded onto miRISC, Argonaute cleaves the second strand which then dissociates from the complex. In its active form as part of miRISC, a miRNA is single-stranded.

You are both right, it is a matter of timing.

Last edited by Jon Moulton; 01-24-2008 at 03:19 PM.
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