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12-06-2007, 02:30 PM
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 tranfecting HL-1 cells (cardiomyocytes) This is a relatively high maintenance cell line that acquires a 'beating' cardiac myocyte phenotype when near confluence. At issue is when to transfect -- should I wait until the phenotype of interest (beating) is present even though confluence is at ~90-95%? Or, would it be best to plate and then transfect shortly after (within 6-12 hours) on a 6 well plate when confluency is ~60-70%?        |
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| cardiomyocytes , cells , hl1 , tranfecting |
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