Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   RNAi and SiRNA Forum (http://www.molecularstation.com/forum/rnai-sirna-forum/)
-   -   miRNA specificity controls: paper (http://www.molecularstation.com/forum/rnai-sirna-forum/1818-mirna-specificity-controls-paper.html)

Jon Moulton 07-24-2007 02:23 PM

miRNA specificity controls: paper
 
Knockdowns of miRNA activity have generally used oligos targeting the miRNA guide strand. A Morpholino oligo targeting an miRNA guide strand can interfere with the activity of the miRNA. It is difficult to control for the specificity of the knockdown when using this technique alone. However, Morpholinos targeting the nucleolytic processing sites of an immature miRNA can prevent maturation of the miRNA. This allows sets of nonoverlapping Morpholino oligos targeting a primary miRNA to be used as specificity controls; if two non-overlapping oligos targeting the same miRNA produce the same phenotype, this supports the hypothesis that the phenotype is due to knocking down the activity of the targeted miRNA and not due to an off-target effect. These techniques are explored in the following paper:

Kloosterman WP, Lagendijk AK, Ketting RF, Moulton JD, Plasterk RHA. Targeted inhibition of miRNA maturation with morpholinos reveals a role for miR-375 in pancreatic islet development. PLoS Biol. 2007;5(8): e203.

Available online at the PLoS Biology website.

postdock 01-24-2008 05:06 PM

Re: miRNA specificity controls: paper
 
Awesome paper, are you working on Morpholinos?

Also do you know if there are any new products/kits using this technology?
Is there increased efficiency other siRNA/RNAis?

very cool stuff:thumbs:

Jon Moulton 01-28-2008 08:44 PM

Re: miRNA specificity controls: paper
 
Hi Postdock,

Yes, I work for Gene Tools, so I work with Morpholinos. I'm a coauthor on the paper cited at the start of this thread.

For new products, if you are working with animals you might be interested in the Vivo-Morpholinos: www<dot>gene-tools<dot>com/vivomorpholinos

Regarding a comparison with siRNA, see this paper: www<dot>gene-tools<dot>com/files/Summerton2007siRNAcompare.pdf

Replace each <dot> with a "." to make the URLs work.


All times are GMT. The time now is 03:40 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.09102 seconds with 11 queries