Go Back   Molecular Biology Forum > Molecular Research Topics Forum > RNA Techniques Forum > RNAi and SiRNA Forum
Register Blogs FAQ Members List Calendar Science Groups New! Arcade Search Today's Posts Mark Forums Read

RNAi and SiRNA Forum Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.

rnai Videos

· · ·
short-interfering-rna-sirna-video Video



miRNA target

RNAi and SiRNA Forum

Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.



Register Molecular Biology Forums
Reply
 
LinkBack Thread Tools Display Modes
  #1 (permalink)  
Old 06-14-2007, 04:20 PM
Pipette Filler
Points: 612, Level: 12Points: 612, Level: 12Points: 612, Level: 12
Activity: 0%Activity: 0%Activity: 0%
 

Join Date: Jun 2007
Posts: 1
andy RSS Feed
Default miRNA target

Dear all:
I recently found in the 3’-untranslated region (3’-UTR) of mRNA for my rat gene of interest a putative target for micro-RNA (miRNA). I did not find such miRNA among those that are currently characterized. I believe this may be because this specific miRNA is not yet identified. I want to extract total small RNAs. However, I do not know how to isolate from this mixture of small RNAs the one that may be specific for the target sequence in 3’-UTR of my gene. Hybridization, annealing, kits, chips? Is there something that you can propose? Please, help.

Thank you
Andy
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
Alt Today
Advertising
Google Adsense
 
This advertising will not be shown
in this way to registered members.
Register your free account today
and become a member on
Molecular Biology Forum
Standard Sponsored Links

Reply

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On
Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
miRNA Databases molecule2005 RNAi and SiRNA Forum 0 02-26-2008 07:48 AM
Another miRNA Role aftabac Science News and Views 0 01-31-2008 04:14 AM
miRNA specificity controls: paper Jon Moulton RNAi and SiRNA Forum 2 01-28-2008 07:44 PM
miRNA yfsq RNA Techniques Forum 3 11-06-2007 03:30 PM
what are the differences between siRNA and miRNA? phone RNAi and SiRNA Forum 2 05-30-2007 12:23 AM


All times are GMT. The time now is 11:19 PM.


Powered by vBulletin® Version 3.7.1
Copyright ©2000 - 2008, Jelsoft Enterprises Ltd.
Copyright 2005-2007 Molecular Station | All Rights Reserved