		Molecular Biology Forum > Molecular Research Topics Forum > RNA Techniques Forum > RNAi and SiRNA Forum
siRNA and stable transfection of stem cells

RNAi and SiRNA Forum Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.

rnai Videos

short-interfering-rna-sirna-video Short Interfering RNA SiRNA Video. The basics of the mechanism of siRNA are explained in this video shown in Parkinsons Disease Revision PPT at the IX Congress of Physiology Students 2005. The Process of Short Interfering RNA.

siRNA and stable transfection of stem cells

RNAi and SiRNA Forum

Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.

LinkBack

Thread Tools

Display Modes

#1 (permalink)

04-26-2007, 06:55 AM

nanaz

Pipette Filler

Join Date: Apr 2007

Posts: 1

siRNA and stable transfection of stem cells

Hi
I am looking for the best available vector to transfect my mouse ESCs with some siRNAs (rather shRNA). Have so far looked at the invitrogen and clontech as options and would preferrably like to do an inducer system. Can any one tell me what are the difficulties and which is the best vector known??
I am told stem cells tend to cause promotor silencing and so I should be looking at several promotors not that I fully get it but can you help?

Thanks

Today

Advertising
Google Adsense

This advertising will not be shown

in this way to registered members.

and become a member on

Molecular Biology Forum

Sponsored Links

« RNAi technology has opened up a whole new approach | Morpholino, siRNA, and S-DNA Compared »

Thread Tools
Show Printable Version Email this Page
Display Modes
Linear Mode Switch to Hybrid Mode Switch to Threaded Mode

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is Off Trackbacks are On Pingbacks are On Refbacks are On

Forum Jump

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Stable siRNA transfection	Labnovice	RNAi and SiRNA Forum	0	04-20-2008 09:57 PM
Stable Transfection with siRNA and RNAi	kiki06	RNAi and SiRNA Forum	3	07-13-2007 06:21 PM

All times are GMT. The time now is 02:54 AM.

Member Login

siRNA and stable transfection of stem cells

RNAi and SiRNA Forum

Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.