Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > RNA Techniques Forum > RNAi and SiRNA Forum
Register Search Today's Posts Mark Forums Read

RNAi and SiRNA Forum Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.


MicroRNA & RNA interference..please explain?

MicroRNA & RNA interference..please explain? - RNAi and SiRNA Forum

MicroRNA & RNA interference..please explain? - Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 03-30-2007, 09:20 PM
Pipette Filler
Points: 1,346, Level: 21 Points: 1,346, Level: 21 Points: 1,346, Level: 21
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Mar 2007
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default MicroRNA & RNA interference..please explain?



I do not understand what exactly microRNA and RNA interference are. It seems too complicated in the text book...can someone explain this to me very simply.
Reply With Quote
  #2  
Old 03-30-2007, 09:21 PM
Pipette Filler
Points: 1,325, Level: 21 Points: 1,325, Level: 21 Points: 1,325, Level: 21
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Mar 2007
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default

microRNAs are very short (about 25 nucleotides) they are never translated into protein, instead they form stem-loop structures. They are thought to control the expression of other genes... they bind to complementary sequences in normal messenger RNA and target them for degradation.

RNAi basically utilizes this endogenous pathway... it is a technique for silencing the expression of target genes.

These are the very basics, but obviously the mechansms by which they occur are detailed. Ambion.com has a very good explanation of RNAi becaus ehtey sell a lot of products for doing it.
Reply With Quote
Reply

Tags
explain , interferenceplease , microrna , rna


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Can someone explain RNA interference to me in simplified terms? imaginexpeace RNAi and SiRNA Forum 0 06-08-2009 10:27 AM
qPCR NEWS April 2009 - focus on microRNA normalisation Editor www.Gene-Quantification.info Protocols and Methods Forum 0 04-29-2009 02:18 PM
qPCR Newsletter September 2008 Editor www.Gene-Quantification.info Protocols and Methods Forum 0 09-29-2008 10:06 AM
qPCR NEWSLETTER - August 2007 Editor www.Gene-Quantification.info Protocols and Methods Forum 0 08-09-2007 02:40 PM
qPCR NEWS - MAy 2006 - Quantitative real-time RT-PCR and microRNA Editor www.Gene-Quantification.info Protocols and Methods Forum 0 05-30-2006 02:52 PM


All times are GMT. The time now is 05:18 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11821 seconds with 16 queries