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RNAi and SiRNA Forum Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.


What's the difference between miRNA and

What's the difference between miRNA and - RNAi and SiRNA Forum

What's the difference between miRNA and - Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.


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Old 03-30-2007, 09:20 PM
Pipette Filler
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Default What's the difference between miRNA and



siRNA? And how would you prove that a sample has miRNA or siRNA
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Old 03-30-2007, 09:20 PM
Pipette Filler
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siRNA is 20-25 nucleotide-long double-stranded RNA molecules

miRNA are single-stranded RNA molecules of about 21-23 nucleotides in length thought to regulate the expression of other genes

So siRNA is double stranded
miRNA is single stranded
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Old 03-30-2007, 09:23 PM
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Essentially the difference is partially to do with the source of the RNA interference (RNAi), and partly down to the structure of the final molecules. Sort interfering RNA (siRNA) is exogenous (originates from outside of the cell), whereas micro RNA (miRNA) is endogenous (originates from within the cell.) Both siRNA and miRNA are non-coding and are primarily targeted at gene repression.

As miRNA are endogenous they are produced through protein/RNA interaction within the cell expression them.

They can be told apart as siRNA molecules exist in a duplex, whereas miRNA are single stranded. They can be further told apart through sequencing, miRNA tend to be less specific than siRNA being only partially complimentary to the RNA being knocked down.
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