Go Back   Molecular Biology Forum > Molecular Research Topics Forum > RNA Techniques Forum > RNAi and SiRNA Forum
Register Blogs FAQ Members List Calendar Science Groups New! Arcade Search Today's Posts Mark Forums Read

RNAi and SiRNA Forum Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.


rnai Videos
short-interfering-rna-sirna-video
short-interfering-rna-sirna-video Short Interfering RNA SiRNA Video. The basics of the mechanism of siRNA are explained in this video shown in Parkinsons Disease Revision PPT at the IX Congress of Physiology Students 2005. The Process of Short Interfering RNA.


What's the difference between miRNA and

RNAi and SiRNA Forum

Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.



Register Molecular Biology Forums
Reply
 
LinkBack Thread Tools Display Modes
  #1 (permalink)  
Old 03-30-2007, 09:20 PM
Pipette Filler
Points: 771, Level: 15Points: 771, Level: 15Points: 771, Level: 15
Activity: 0%Activity: 0%Activity: 0%
 

Join Date: Mar 2007
Posts: 1
Luis f RSS Feed
Default What's the difference between miRNA and

siRNA? And how would you prove that a sample has miRNA or siRNA
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
Alt Today
Advertising
Google Adsense
 
This advertising will not be shown
in this way to registered members.
Register your free account today
and become a member on
Molecular Biology Forum
Standard Sponsored Links

  #2 (permalink)  
Old 03-30-2007, 09:20 PM
Pipette Filler
Points: 753, Level: 15Points: 753, Level: 15Points: 753, Level: 15
Activity: 0%Activity: 0%Activity: 0%
 

Join Date: Mar 2007
Posts: 1
-Eugenious- RSS Feed
Default

siRNA is 20-25 nucleotide-long double-stranded RNA molecules

miRNA are single-stranded RNA molecules of about 21-23 nucleotides in length thought to regulate the expression of other genes

So siRNA is double stranded
miRNA is single stranded
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
  #3 (permalink)  
Old 03-30-2007, 09:23 PM
Pipette Filler
Points: 753, Level: 15Points: 753, Level: 15Points: 753, Level: 15
Activity: 0%Activity: 0%Activity: 0%
 

Join Date: Mar 2007
Posts: 1
alexjcharlton RSS Feed
Default

Essentially the difference is partially to do with the source of the RNA interference (RNAi), and partly down to the structure of the final molecules. Sort interfering RNA (siRNA) is exogenous (originates from outside of the cell), whereas micro RNA (miRNA) is endogenous (originates from within the cell.) Both siRNA and miRNA are non-coding and are primarily targeted at gene repression.

As miRNA are endogenous they are produced through protein/RNA interaction within the cell expression them.

They can be told apart as siRNA molecules exist in a duplex, whereas miRNA are single stranded. They can be further told apart through sequencing, miRNA tend to be less specific than siRNA being only partially complimentary to the RNA being knocked down.
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
Reply

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On
Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Another miRNA Role aftabac Science News and Views 0 01-31-2008 04:14 AM
miRNA specificity controls: paper Jon Moulton RNAi and SiRNA Forum 2 01-28-2008 07:44 PM
miRNA yfsq RNA Techniques Forum 3 11-06-2007 03:30 PM
miRNA target andy RNAi and SiRNA Forum 0 06-14-2007 04:20 PM
what are the differences between siRNA and miRNA? phone RNAi and SiRNA Forum 2 05-30-2007 12:23 AM


All times are GMT. The time now is 02:47 AM.


Powered by vBulletin® Version 3.7.1
Copyright ©2000 - 2008, Jelsoft Enterprises Ltd.
Copyright 2005-2007 Molecular Station | All Rights Reserved