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#1
10-15-2008, 11:04 PM
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 Intronic splice regulatory mutation "treated" with Morpholino oligo A mutation was found in intron 1 of the beta fibrinogen gene of a patient suffering afibrogenemia. A construct bearing the mutant of the fibrinogen beta gene was put into COS-7 cells. Using Endo-Porter to deliver a Morpholino into the cultured COS-7 cells corrected the mutant splicing to wild-type splicing. The Morpholino blocked the binding of a splice-regulatory protein to the mutant splice site. Davis RL, Homer VM, George PM, Brennan SO. A deep intronic mutation in FGB creates a consensus exonic splicing enhancer motif that results in afibrinogenemia caused by aberrant mRNA splicing, which can be corrected in vitro with antisense oligonucleotide treatment. Hum Mutat. 2008 Oct 13. [Epub ahead of print] [Only registered users see links. ]  |
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| intronic , morpholino , mutation , oligo , regulatory , splice , splice intron morpholino , treated |
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