Intronic splice regulatory mutation "treated" with Morpholino oligo
A mutation was found in intron 1 of the beta fibrinogen gene of a patient suffering afibrogenemia. A construct bearing the mutant of the fibrinogen beta gene was put into COS-7 cells. Using Endo-Porter to deliver a Morpholino into the cultured COS-7 cells corrected the mutant splicing to wild-type splicing. The Morpholino blocked the binding of a splice-regulatory protein to the mutant splice site.
Davis RL, Homer VM, George PM, Brennan SO. A deep intronic mutation in FGB creates a consensus exonic splicing enhancer motif that results in afibrinogenemia caused by aberrant mRNA splicing, which can be corrected in vitro with antisense oligonucleotide treatment. Hum Mutat. 2008 Oct 13. [Epub ahead of print] [Only registered users see links. ]
__________________ Jon D. Moulton, Ph.D.
Gene Tools, LLC [Only registered users see links. ]