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beenabijoy 06-19-2006 03:45 PM

RNA ladder
 
My RNA lenth is 30 mer. I want to custom generate an RNA laddr with Ribonuclease A digestion of the 30mer to creat 4, 6 18 and 26 mers. How can I do that.?

darkwng 06-19-2006 06:22 PM

Re: RNA ladder
 
Quote:

Originally Posted by beenabijoy
My RNA lenth is 30 mer. I want to custom generate an RNA laddr with Ribonuclease A digestion of the 30mer to creat 4, 6 18 and 26 mers. How can I do that.?

Interesting idea, however i know that Rnase A / T1 and other RNases do not cut specifically like Restriction Enzymes. So the only thing you could try, but I doubt it would work is do a Time Course of digestion.

ie try cutting it for a few seconds to a minute. I still doubt you can know how long it will need and what fragment size you will get so....:unsure:

the cheapest and easiest way to get an RNA ladder is to buy one. they are quite cheap and your supervisor should pay for it. I would go that way.

Fermentas has 100-1000 nucleotide RNA ladders
[Only registered and activated users can see links. Click Here To Register...]

Although the best one I found is this RNA ladder with Fragment sizes (base): 30,26,22,18,14 nucleotides.

[Only registered and activated users can see links. Click Here To Register...]


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