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Gene knockdowns in the wasp Nasonia vitripennis

Gene knockdowns in the wasp Nasonia vitripennis - RNA Techniques Forum

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Old 03-07-2014, 05:59 PM
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Post Gene knockdowns in the wasp Nasonia vitripennis



This paper describes gene knockdowns in the wasp Nasonia vitripennis by Morpholino antisense oligos, delivered by microinjection.

Dual mode of embryonic development is highlighted by expression and function of Nasonia pair-rule genes. Rosenberg MI, Brent AE, Payre F, Desplan C. eLife. 2014;3:e01440 doi:10.7554/eLife.01440

"Although parental RNAi in Nasonia is effective for maternal and early zygotic genes ..., it often does not provide significant knockdown of later-acting genes. To overcome this limitation, we designed an Nv eve morpholino overlapping the translation start site, as well as one directed at the exon-intron junction in the homeobox. These two independent morpholinos are expected to disrupt Nv eve activity and indeed result in comparable phenotypes."

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