Antisense oligonucleotides (ASOs) and ss-siRNA
 

Hi everyone

Currently, single-stranded small interfering RNAs (ss-siRNAs) are used instead of double strand siRNA. In nucleotide sequence, the ss-siRNA is similar with antisense oligonucleotides (ASOs)??? Could you please explain what is difference between ASOs and ss-siRNAs?

Alle

Re: Antisense oligonucleotides (ASOs) and ss-siRNA
 

An siRNA oligo uses RISC for its activity. An antisense oligo either activates RNase-H activity or sterically blocks its target.