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DIG labelled RNA marker is appearing on gel?

DIG labelled RNA marker is appearing on gel? - RNA Techniques Forum

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Old 07-08-2012, 08:29 AM
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Default DIG labelled RNA marker is appearing on gel?



Dear All,
I am using Roche RNA molecular weight marker I (DIG labelled). I could not see marker bands on the gel, although I loaded 100 ng per lane. I could see very faint bands on the agarose gel but not on formaldehyde agarose gel which is preffered by Roche. I am using the loading buffer as recommended by Roche, only difference is of formamide which was not deionized.

Any suggestion would be highly appreciated.

Regards,

Shahid
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