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RNA Techniques Forum Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


Rna-rna emsa

Rna-rna emsa - RNA Techniques Forum

Rna-rna emsa - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


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Old 05-23-2012, 01:57 AM
Pipette Filler
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Default Rna-rna emsa



Hi

I need to test if a sRNA and mRNA interact, I'm using a RNA-RNA EMSA protocol published in Molecular Microbiology (2007) 64(5), 1260–1273, but I don't see a shift in the migration of sRNA (which is radiolabeled) in presence of different concentrations of mRNA . Both RNAs are of 100bp, I have used 5 to 9% native polyacrylamide gels. So if somebody has recomendations to detect the interaction, i will be grateful.
Kind regards
Luis

Last edited by luisfemu; 05-23-2012 at 01:58 AM.
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Old 05-23-2012, 02:09 AM
Pipette Filler
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Default Re: Rna-rna emsa

Hi

I need to test if a sRNA and mRNA interact, I'm using a RNA-RNA EMSA protocol published in Molecular Microbiology (2007) 64(5), 12601273, but I don't see a shift in the migration of sRNA (which is radiolabeled) in presence of different concentrations of mRNA and positive control that is an antisense of sRNA. Both RNAs are of 100bp, I have used 5 to 9% native polyacrylamide gels. So if somebody has recomendations to detect the interaction, i will be grateful.
Kind regards
Luis[/QUOTE]
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