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RNA Techniques Forum Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


which RNA? which extraction method? which kit?

which RNA? which extraction method? which kit? - RNA Techniques Forum

which RNA? which extraction method? which kit? - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


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Old 04-11-2012, 01:58 PM
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Default which RNA? which extraction method? which kit?



I'm working with HepG2 cells and want to extract RNA for both microchip gene expression analysis, and for follow up RT-PCR.
Should I extract total RNA or mRNA? (seems different kits used for each)
Should I use "RNA stability products" - advertised to maintain fidelity of gene expression?
And products people have used and are happy with? Biorad, Sigma, and Qiogen both seem to offer lots of different products/kits - feels overwhelming!!
thx!
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