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-   -   which RNA? which extraction method? which kit? (http://www.molecularstation.com/forum/rna-techniques-forum/85114-rna-extraction-method-kit.html)

t.christides 04-11-2012 01:58 PM

which RNA? which extraction method? which kit?
 
I'm working with HepG2 cells and want to extract RNA for both microchip gene expression analysis, and for follow up RT-PCR.
Should I extract total RNA or mRNA? (seems different kits used for each)
Should I use "RNA stability products" - advertised to maintain fidelity of gene expression?
And products people have used and are happy with? Biorad, Sigma, and Qiogen both seem to offer lots of different products/kits - feels overwhelming!!
thx!
:help:


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