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Yellow pellet after isopropanol precipitation
During RNA extraction of larval stage nematodes, upon addition of isopropanol the solution turned yellow and after centrifuging, the pellet was yellow too. After washing with 75% EtOh the yellow pellet remained the same colour and even dissolved as yellow in H2O. I think it must be the isopropanol. The 260/280 values were ok but the 260/230 values were very very low making me think the isopropanol retained salts or something with the pellet. Any idea why this is and if there's anything I can do to fix it?
Re: Yellow pellet after isopropanol precipitation
I'm facing the same problem during DNA extraction. Are you using Phenol:Chlorophorm:IAA in your extraction method. I get yellow aqueous upper layer when this extraction mixture not stored or prepared properly. But I still haven't figured out the problem.
Any one can give suggestions ??
|isopropanol , pellet , precipitation , yellow|
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