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concentration of RNA

concentration of RNA - RNA Techniques Forum

concentration of RNA - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


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Old 03-15-2011, 03:21 PM
Pipette Filler
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Default concentration of RNA



I have extracted RNA from tomato using seed and pericarp. For the seed, I got the RNA concentration around 45-55ng/ul (in 40ul) and for pericarp I got 27-35ng/ul (in 40ul). Are these concentration low or high?? I have ran the gel to look at my RNA and the bands are very clear and quite thick. And then I have to do the DNase free treatment which decrease more of my RNA concentration and then proceed to the cDNA synthesis where I have to use around 1ug of RNA . I'm not sure whether I have enough RNA to synthesize my cDNA. Any suggestion on how to increase my RNA concentration and how can I synthesize my cDNA using these samples...
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Old 04-01-2012, 05:19 AM
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Default Re: concentration of RNA

how do u isolate RNA in the first place?
biozol??? if so..in my experience u dont need to treat DNA because it's already separated in a buffy layer after chloroform addition
if u using columns, they'r also designed to remove DNA through the purification procedure..
so i need to know how did u isolate ur RNA first..
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Old 04-20-2012, 06:15 AM
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Default レッドストーン rmt

Basically burning your brand on their memory is ones own advertising objective.
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Old 04-20-2012, 06:16 AM
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Default Re: concentration of RNA

Still other people might have a degree in one discipline but wish to pursue one thing they now develop an interest in.
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