concentration of RNA

[ifhmn]

I have extracted RNA from tomato using seed and pericarp. For the seed, I got the RNA concentration around 45-55ng/ul (in 40ul) and for pericarp I got 27-35ng/ul (in 40ul). Are these concentration low or high?? I have ran the gel to look at my RNA and the bands are very clear and quite thick. And then I have to do the DNase free treatment which decrease more of my RNA concentration and then proceed to the cDNA synthesis where I have to use around 1ug of RNA . I'm not sure whether I have enough RNA to synthesize my cDNA. Any suggestion on how to increase my RNA concentration and how can I synthesize my cDNA using these samples...

[Dr. happy]

how do u isolate RNA in the first place?
biozol??? if so..in my experience u dont need to treat DNA because it's already separated in a buffy layer after chloroform addition
if u using columns, they'r also designed to remove DNA through the purification procedure..
so i need to know how did u isolate ur RNA first..

[TERARMT]

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[TERARMT]

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