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ifhmn 03-15-2011 03:21 PM

concentration of RNA
 
I have extracted RNA from tomato using seed and pericarp. For the seed, I got the RNA concentration around 45-55ng/ul (in 40ul) and for pericarp I got 27-35ng/ul (in 40ul). Are these concentration low or high?? I have ran the gel to look at my RNA and the bands are very clear and quite thick. And then I have to do the DNase free treatment which decrease more of my RNA concentration and then proceed to the cDNA synthesis where I have to use around 1ug of RNA . I'm not sure whether I have enough RNA to synthesize my cDNA. Any suggestion on how to increase my RNA concentration and how can I synthesize my cDNA using these samples...

Dr. happy 04-01-2012 05:19 AM

Re: concentration of RNA
 
how do u isolate RNA in the first place?
biozol??? if so..in my experience u dont need to treat DNA because it's already separated in a buffy layer after chloroform addition
if u using columns, they'r also designed to remove DNA through the purification procedure..
so i need to know how did u isolate ur RNA first..

TERARMT 04-20-2012 06:15 AM

レッドストーン rmt
 
Basically burning your brand on their memory is ones own advertising objective.

TERARMT 04-20-2012 06:16 AM

Re: concentration of RNA
 
Still other people might have a degree in one discipline but wish to pursue one thing they now develop an interest in.


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