Hi everybody! Do you have any experience with commercial kits for rRNA removal from total RNA prior to RNA-seq? I was considering some of the Invitrogen kits, but it is difficult to decide which one. I went through all the Invitrogen web, but the important issues are not obvious from the web page. The most important question for me is whether the sequences of the rRNA-specific probes are available anywhere? It is crucial for me to decide which kit should I use, since I work with non-model organism for which the kit definitely was not tested.
And furthermore, what is the difference between RiboMinus™ XXX Transcriptome Isolation Kit and RiboMinus™ XXX Kit for RNA-Seq? (XXX stands for an organism). And what is the difference between RiboMinus™ Human⁄Mouse Transcriptome Isolation Kit 6 reactions (K1550-01) and RiboMinus™ Transcriptome Isolation Kit (Human⁄Mouse) 6 reactions (K1550-02)? Both are for transcriptome isolation from Human/Mouse, both are for six reactions; just different price?
Thanks in advance for any tips/advices!