hello microarray experts,
i'm trying to run an microarray experiment with knock-out embryos. my desired genotype is Tg1/Tg1;Tg2/+ using Tg1/+;Tg2/+ and Tg1/+;+/+ as parents (
Tg1/Tg1 is non-viable so I can't use it as parents). Using this mating strategy, the chance I will get a embryo with a desired genotype is 1/8 in the whole litter, which means at best, I will get 1-2 embryos. I'm not sure if this is enough for a gene array experiment. so i'm considering to save the cells after sorting in RNAlater or progressing all the way to RNA extraction, and saving the RNA; then once enough cells or RNA has been accumulated then I will send it off for microarray. What would be the best way to accumulate cells/RNA for samples to be collected over time due to difficult-to-obtain tissues?
thanks so much for your response!!!