Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > RNA Techniques Forum
Register Search Today's Posts Mark Forums Read

RNA Techniques Forum Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


RNA/cell accumulation of difficult-to-obtain tissue

RNA/cell accumulation of difficult-to-obtain tissue - RNA Techniques Forum

RNA/cell accumulation of difficult-to-obtain tissue - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 01-16-2011, 11:21 PM
Pipette Filler
Points: 283, Level: 5 Points: 283, Level: 5 Points: 283, Level: 5
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2010
Posts: 3
Thanks: 1
Thanked 0 Times in 0 Posts
Default RNA/cell accumulation of difficult-to-obtain tissue



hello microarray experts,

i'm trying to run an microarray experiment with knock-out embryos. my desired genotype is Tg1/Tg1;Tg2/+ using Tg1/+;Tg2/+ and Tg1/+;+/+ as parents (
Tg1/Tg1 is non-viable so I can't use it as parents). Using this mating strategy, the chance I will get a embryo with a desired genotype is 1/8 in the whole litter, which means at best, I will get 1-2 embryos. I'm not sure if this is enough for a gene array experiment. so i'm considering to save the cells after sorting in RNAlater or progressing all the way to RNA extraction, and saving the RNA; then once enough cells or RNA has been accumulated then I will send it off for microarray. What would be the best way to accumulate cells/RNA for samples to be collected over time due to difficult-to-obtain tissues?

thanks so much for your response!!!

- minnie
Reply With Quote
Reply

Tags
accumulation , difficulttoobtain , rna or cell , tissue


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Tissue Engineering & Stem Cell Research 2005 Andrew Oliver Protocols and Methods Forum 0 12-02-2004 11:38 PM


All times are GMT. The time now is 02:58 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11057 seconds with 16 queries