|Register||Search||Today's Posts||Mark Forums Read|
|RNA Techniques Forum Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.|
| ||LinkBack||Thread Tools||Display Modes|
RNA/cell accumulation of difficult-to-obtain tissue
hello microarray experts,
i'm trying to run an microarray experiment with knock-out embryos. my desired genotype is Tg1/Tg1;Tg2/+ using Tg1/+;Tg2/+ and Tg1/+;+/+ as parents (
Tg1/Tg1 is non-viable so I can't use it as parents). Using this mating strategy, the chance I will get a embryo with a desired genotype is 1/8 in the whole litter, which means at best, I will get 1-2 embryos. I'm not sure if this is enough for a gene array experiment. so i'm considering to save the cells after sorting in RNAlater or progressing all the way to RNA extraction, and saving the RNA; then once enough cells or RNA has been accumulated then I will send it off for microarray. What would be the best way to accumulate cells/RNA for samples to be collected over time due to difficult-to-obtain tissues?
thanks so much for your response!!!
|accumulation , difficulttoobtain , rna or cell , tissue|
|Thread||Thread Starter||Forum||Replies||Last Post|
|Tissue Engineering & Stem Cell Research 2005||Andrew Oliver||Protocols and Methods Forum||0||12-02-2004 10:38 PM|