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Help with RNA quantification please :)

Help with RNA quantification please :) - RNA Techniques Forum

Help with RNA quantification please :) - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


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Old 07-27-2010, 04:43 PM
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Default Help with RNA quantification please :)



Hi.

I'm looking at gene expression and so have run a reverse transcriptase pcr on my RNA samples. My supervisor said something about running a lambda ladder to do a rough analysis of expression. I can't do a blot or qrt-pcr as I have limited time and money for my project.
Anyway, I was wondering if I should do this lamda ladder straight from the rt-pcr samples or if I needed to somehow purify the target? I have tried to ask my supervisor but he's away at the minute () so hopefully someone on here can help?

Thanks
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help & suggestions plz , quantification , rna


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