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How to get RNA sample denature to get a nice band on electrophoresis gel?

How to get RNA sample denature to get a nice band on electrophoresis gel? - RNA Techniques Forum

How to get RNA sample denature to get a nice band on electrophoresis gel? - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


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Old 04-02-2010, 08:38 AM
Pipette Filler
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Default How to get RNA sample denature to get a nice band on electrophoresis gel?



Hi everyone,
I am working with single strand plant virus RNA. I have had run many gels, but I am not able to get a nice band of my sample. I have tried agarose gel and page. Urea doesnt denature my RNA sample at all, so im trying to run it on agarose gel. It looks like my RNA sample doesnt like to lose its secondary structure.

Does anyone knows a good denaturing reagent that can help me denature my sample to get a nice clear band?

Thank u
Sara
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Old 04-07-2011, 04:42 PM
Pipette Filler
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Default Re: How to get RNA sample denature to get a nice band on electrophoresis gel?

usually after adding the loading buffer you should heat the mixture at 65 C during 10 min and after put on ice... do not be worry since the loading buffer contain formamide that stabilize 100 % the RNA
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