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-   -   How to get RNA sample denature to get a nice band on electrophoresis gel? (http://www.molecularstation.com/forum/rna-techniques-forum/72708-how-get-rna-sample-denature-get-nice-band-electrophoresis-gel.html)

Sara Fan 04-02-2010 08:38 AM

How to get RNA sample denature to get a nice band on electrophoresis gel?
 
Hi everyone,
I am working with single strand plant virus RNA. I have had run many gels, but I am not able to get a nice band of my sample. I have tried agarose gel and page. Urea doesnt denature my RNA sample at all, so im trying to run it on agarose gel. It looks like my RNA sample doesnt like to lose its secondary structure.

Does anyone knows a good denaturing reagent that can help me denature my sample to get a nice clear band?

Thank u
Sara

Ramiro 04-07-2011 04:42 PM

Re: How to get RNA sample denature to get a nice band on electrophoresis gel?
 
usually after adding the loading buffer you should heat the mixture at 65 C during 10 min and after put on ice... do not be worry since the loading buffer contain formamide that stabilize 100 % the RNA :)


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