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Morpholinos to make mice dystrophic then fix them

Morpholinos to make mice dystrophic then fix them - RNA Techniques Forum

Morpholinos to make mice dystrophic then fix them - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


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Old 03-25-2010, 05:34 PM
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Default Morpholinos to make mice dystrophic then fix them



Fletcher S, Adams AM, Johnsen RD, Greer K, Moulton HM, Wilton SD. Dystrophin Isoform Induction In Vivo by Antisense-mediated Alternative Splicing. Mol Ther. 2010 Mar 23. [Epub ahead of print]
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No animal model for a mutation? Wilton et al. use one Morpholino to alter splicing and phenocopy a mutation at the RNA level, then another Morpholino to remove another exon and restore the reading frame. The resulting protein has the same structure as one produced by a mutant lacking an exon that is treated with a frame-correcting oligo.
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