Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > RNA Techniques Forum
Register Search Today's Posts Mark Forums Read

RNA Techniques Forum Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


Best Method to identify all RNA sequences from Extraction

Best Method to identify all RNA sequences from Extraction - RNA Techniques Forum

Best Method to identify all RNA sequences from Extraction - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 01-12-2010, 09:25 PM
Pipette Filler
Points: 36, Level: 1 Points: 36, Level: 1 Points: 36, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2010
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Default Best Method to identify all RNA sequences from Extraction



Apologies for cross-posting in the RNA forum...

I am trying to find the most elegant way to identify all of the RNA that I obtain from an extraction - from protein produced in E. coli. My idea involves using a 3' RNA tailing kit to poly adenylate all RNA and then find some way to ligate stuff to the 5' ends and then use RT-PCR. Any other ideas?
Reply With Quote
Reply

Tags
extraction , identify , method , rna , sequences


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Best method to identify all RNA sequences from extraction 8peSht RNA Forum 1 01-13-2010 12:33 AM
DNA extraction Method from Blood molgen DNA Forum 4 10-26-2009 04:34 PM
Plant Protein Extraction the Hard Way danfive Protein Science 0 04-24-2008 08:53 PM
clustering/distances for unrelated sequences Thomas Isenbarger Protocols and Methods Forum 3 12-11-2004 08:13 PM
Scientific method (was Re: Fusion In Utah (again)) Bill Vajk Chemistry Forum 12 09-29-2003 08:30 PM


All times are GMT. The time now is 11:12 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.10938 seconds with 16 queries