Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > RNA Techniques Forum
Register Search Today's Posts Mark Forums Read

RNA Techniques Forum Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


mRNA quality check

mRNA quality check - RNA Techniques Forum

mRNA quality check - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 12-22-2009, 07:58 PM
Pipette Filler
Points: 152, Level: 3 Points: 152, Level: 3 Points: 152, Level: 3
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2009
Posts: 1
Thanks: 1
Thanked 0 Times in 0 Posts
Exclamation mRNA quality check



Hello.. I need to know how to check for the integrity of mRNA. I recently have ordered Poly(A) RNA Of normal human bladder tissue & it has been brought to us (overseas) on wet ice. So I just need to make sure that this RNA is really intact.. How can i do so?
Reply With Quote
  #2  
Old 12-23-2009, 06:05 PM
Jon Moulton's Avatar
Post-Doc
Points: 8,875, Level: 65 Points: 8,875, Level: 65 Points: 8,875, Level: 65
Activity: 99% Activity: 99% Activity: 99%
 
Join Date: May 2007
Location: Corvallis, OR, USA
Posts: 256
Thanks: 3
Thanked 15 Times in 15 Posts
Default Re: mRNA quality check

Gel electrophoresis can tell you if you have a strong band at the expected mass. Detecting a population of cleavage products would be more difficult, but the lack of a lower-molecular-mass smear following your strong expected band would suggest that you don't have very much degraded sequence.
Reply With Quote
The Following User Says Thank You to Jon Moulton For This Useful Post:
maha.elzeiry (12-24-2009)
Reply

Tags
check , mrna , quality


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
How to check quality of RNA for microarray analysis alexmony RNA Techniques Forum 1 11-07-2007 01:38 PM
Rescue mRNA (reply to Sami) (Wilson K. Clements) Wilson Clements Zebrafish Forum 0 05-09-2006 06:52 PM
Rescue mRNA (reply to Sami) Jon D.Moulton Zebrafish Forum 0 05-08-2006 10:29 PM
New Saccharomyces Sequences 09/15/04 SGD Sequences Yeast Forum 0 09-15-2004 03:49 PM


All times are GMT. The time now is 07:23 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12296 seconds with 16 queries