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| RNA Techniques Forum Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols. |
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#1
12-22-2009, 06:58 PM
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 mRNA quality check Hello.. I need to know how to check for the integrity of mRNA. I recently have ordered Poly(A) RNA Of normal human bladder tissue & it has been brought to us (overseas) on wet ice. So I just need to make sure that this RNA is really intact.. How can i do so?  |
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#2
12-23-2009, 05:05 PM
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 Re: mRNA quality check Gel electrophoresis can tell you if you have a strong band at the expected mass. Detecting a population of cleavage products would be more difficult, but the lack of a lower-molecular-mass smear following your strong expected band would suggest that you don't have very much degraded sequence. |
| The Following User Says Thank You to Jon Moulton For This Useful Post: | ||
maha.elzeiry (12-24-2009)
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| check , mrna , quality |
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