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RNA isolation from Arabidopsis sliques

RNA isolation from Arabidopsis sliques - RNA Techniques Forum

RNA isolation from Arabidopsis sliques - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


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Old 10-02-2009, 02:31 PM
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Default RNA isolation from Arabidopsis siliques



Hi,

I tried many a times to isolate whole RNA from Arabidopsis siliques using two different kits, EuroX (roboklon) and peqGOLD TriFast. Both of these kits isolated extremely poor concentration of silique RNA. However, the same kits for other Arabidopsis tissues for example, rosette, flower and root, produced enough concentration and good RNA quality.

Could some body please suggest me how to troubleshoot the problem of RNA isolation from silique tissues. I want to use these RNA for RT-PCR reaction.

Thanks

Last edited by kanil; 10-03-2009 at 09:26 AM.
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