I tried many a times to isolate whole RNA from Arabidopsis siliques
using two different kits, EuroX (roboklon)
and peqGOLD TriFast
. Both of these kits isolated extremely poor concentration of silique RNA. However, the same kits for other Arabidopsis tissues for example, rosette, flower and root, produced enough concentration and good RNA quality.
Could some body please suggest me how to troubleshoot the problem of RNA isolation from silique tissues. I want to use these RNA for RT-PCR reaction.