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{help} RNA degrade

{help} RNA degrade - RNA Techniques Forum

{help} RNA degrade - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


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  #1  
Old 09-25-2009, 07:48 AM
Pipette Filler
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Default {help} RNA degrade



RNA was extracted about 10 months ago. I kept them at -80 C freezer.

now, i do my realtime PCR using those RNA. I confuse, the Ct value >35 cycle

my RNA was degrade? how could we check?
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Old 09-28-2009, 06:21 PM
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Default Re: {help} RNA degrade

check the RNA quality on acrylamide gel.
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Old 09-28-2009, 06:22 PM
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Default Re: {help} RNA degrade

You can also try to amplify a housekeeping gene/transcript.
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