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-   -   {help} RNA degrade (http://www.molecularstation.com/forum/rna-techniques-forum/70731-%7Bhelp%7D-rna-degrade.html)

tomoe 09-25-2009 07:48 AM

{help} RNA degrade
 
RNA was extracted about 10 months ago. I kept them at -80 C freezer.

now, i do my realtime PCR using those RNA. I confuse, the Ct value >35 cycle

my RNA was degrade? how could we check?

danfive 09-28-2009 06:21 PM

Re: {help} RNA degrade
 
check the RNA quality on acrylamide gel.

danfive 09-28-2009 06:22 PM

Re: {help} RNA degrade
 
You can also try to amplify a housekeeping gene/transcript.


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