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Chloroform only clean up step?

Chloroform only clean up step? - RNA Techniques Forum

Chloroform only clean up step? - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


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Old 07-05-2009, 02:30 AM
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Default Chloroform only clean up step?



Hi all,

How common/useful is it to do a chloroform only extraction after first performing an acid-phenol chloroform extraction?

Say i recover the aqueous phase after centrifuging with acid-phenol chloroform, then to this aqueous phase I then add an equal volume of pure chloroform and centrifuge again and recover the aqueous phase before precipitating the RNA
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