RNA and Nanodrop

[Arastho]

Hello everyone,

I have a Nanodrop in my lab that I use for quantify both DNA and RNA after extraction.

Actually I'm concerned about checking RNA integrity without using electrophoresis. (it's offline at the moment! )

Do you know if absorbance profile on the graph of my RNA is modified by degradation?

We use the profile on the graph for analyse DNA. It's very simple but with RNA no one ever tested!

Comments?

Thanks

[Dave L]

Hello Arastho,

The spectrum, "graph" and purity ratios of the Thermo Scientific NanoDropTM Spectrophotometers are very informative for nucleic acid purity of 1 ul samples. Absorbance measurements are not particularly used for sample integrity however. More information may be found at nanodrop.com.

[Barryjohn]

Just thought you should know that after extensive testing the reproducibility on the Nanodrop appears to be rather poor... and it is almost impossible to get reliable readings for protein work... The Implens nanophotometer came out on top by some considerable margin.