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House keeping genes`

House keeping genes` - RNA Techniques Forum

House keeping genes` - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


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Old 05-21-2009, 08:01 PM
Pipette Filler
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Default House keeping genes`



Why is it necessary to co amplify house keeping genes while performing RT PCR?
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Old 05-21-2009, 08:47 PM
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Default Re: House keeping genes`

Hi
housekeeping genes are amplified to standerize the results. the expression of housekeeping gene should be same in all of your testing samples otherwise if RNA concentration may be more in one sample then other then its hard to prove results.
i hope it will help you.

regards
aftab
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