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| Why is it necessary to co amplify house keeping genes while performing RT PCR? |
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#2
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| Hi housekeeping genes are amplified to standerize the results. the expression of housekeeping gene should be same in all of your testing samples otherwise if RNA concentration may be more in one sample then other then its hard to prove results. i hope it will help you. regards aftab |
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| genes` , house , keeping |
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