| | Re: problems extracting RNA from DCs
Dear Alvaro, i work with macrophages in culture bottles or nuncl plaques. I do some like that: i wash the culture with PBS and a put directly the trizol. I colet the trizol and follows the rna extraction protocols. On isopropanol phase i used LPA (linear polyacrilamide) (+- 5ul, per 700 ml of trizol), this reagent drop small nucleic acid like dna less than 20pb. Using this reagent i obtain much quality of my rna amounts. I hope that this information help you.
Álvaro one favor, can you send me the isolation of CDs of any tissue. I like work with gingival tissues.